Fig. 4
Env-specific binding antibodies after Ad-based vaccination of Ad-experienced mice. CB6F1 mice were immunized with 109 vp of the indicated envelope vaccines, with or without immunization with 1010 vp Ad.empty three weeks prior as indicated. F-MuLV-binding antibody titers were analyzed two weeks after completion of the immunization. The data shown were obtained in two independent experiments, using 2–4 mice per group per experiment. Each dot represents one mouse, lines indicate median values, dashed lines indicate the limit of detection. Data were analyzed by Mann–Whitney Rank Sum Test. Significant differences (P < 0.05) compared to mice vaccinated only with the envelope vaccine are indicated by #. Sufficient statistical power was verified (SP = 0.99)