Fig. 3

Induction of regulatory T cells by Ad-based vaccination. CB6F1 mice were immunized with 10⁹ vp Ad.TxnGagL, either as a single immunization or after pre-immunization with 10⁹ vp Ad.GFP or Ad.pIXgp70. Three days after the Ad.TxnGagL immunization, the frequencies of regulatory FoxP3⁺ CD4⁺ T cells (a), of CD44⁺ FoxP3⁺ CD4⁺ T cells (b), of CD62L^low FoxP3⁺ CD4⁺ T cells (c), and of KLRG1⁺ FoxP3⁺ CD4⁺ T cells (d) were analyzed in draining lymph nodes. The data shown were obtained in two independent experiments. Each dot represents one mouse, lines indicate mean values. Data were analyzed by Kruskall–Wallis One Way Analysis of Variance on Ranks and Dunn’s multiple comparison procedure, n.s. not significant (P > 0.05)