Fig. 2
From: Toll-like receptor 3 activation selectively reverses HIV latency in microglial cells
HIV emergence from latency in human microglial cell models. a Genome organization of the HIV lentiviral vector. A fragment of HIV-1pNL4-3, containing Tat, Rev, Env, Vpu and Nef with the reporting gene d2EGFP, is cloned into the pHR’ backbone. The resulted plasmid was used to produce the VSVG HIV particles, as described previously [112]. b Fluorescence microscopy analysis of TNF-α- and HDACi 4b-mediated reactivation of HIV in latently-infected microglial cells [hµglia/HIV (HC69) and (HC01)]. Cells treated with TNF-α (500 pg/mL) or HDACi 4b (30 µM). c FACS analysis 16 h post-treatment. In these, and subsequent FACS profiles, GFP+ cell populations are indicated in green, and the proportion of GFP-expressing cells is indicated in %