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Fig. 8 | Retrovirology

Fig. 8

From: Identification of small molecule modulators of HIV-1 Tat and Rev protein accumulation

Fig. 8

HIV-1 Tat expression can be rescued with proteasome inhibition by MG132. HeLa HIVrtTA∆Mls cells were incubated with 791 (30 µM), 833 (2 µM), or 892 (15 µM) for 24 h in the absence (−) or presence of (+) of Dox. Eight hours prior to harvest, MG132 was added as indicated to a subset of the samples. a Representative blot showing effect of the compounds on HIV-1 Gag and Tat expression in the presence or absence of proteasome inhibitor MG132, relative to DMSO treatment. GAPDH serves as loading control. b Summary of band intensities of HIV-1 p24 Gag and p14 and p16 Tat with each treatment relative to that of the DMSO control normalized to the corresponding GAPDH bands (N ≥ 3). Error bars depict standard error of the mean and *, **, and *** indicate P values ≤ 0.05, 0.01, and 0.001, respectively. c, d To assess effect of compound addition on p53 expression, lysates were prepared from cells treated with 791 (30 µM), 833 (2 µM), or 892 (15 µM) and blots were probed for p53 and GAPDH. Shown are (c) representative blot of results and d summary of quantitation of p53 expression after normalization using GAPDH

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