Fig. 3

Cytotoxicity of NKT cells after FV infection. Mice were infected with FV and were sacrificed at 3 dpi. As control group non-infected mice were used. Single cell suspensions were prepared from bone marrow (white bars) and spleens (gray bars) of mice. Effector functions were measured by the degranulation marker CD107a (a) and apoptosis-inducing FasL (b) and analyzed by flow cytometry. Data were collected from at least five independent experiments and at least eight animals per group. In c, NKT cells from naïve and FV-infected mice were isolated and splenic and bone marrow NKT cells were mixed. NKT cells were co-incubated with CFSE-labeled, FV-transformed tumor cells (FBL-3 cells). Cells were stained for viability and immediately analyzed using flow cytometry. At least four animals per group out of at least two experiments were used for analysis. Mean (±SEM) values of percentages are indicated by bars. Statistically significant differences between groups were analyzed with the Mann–Whitney test and are indicated by single asterisk for p < 0.05 and double asterisk for p < 0.01