Fig. 1
From: Natural killer T cells contribute to the control of acute retroviral infection

Absolute cell numbers and activation of NKT cells. Mice were infected with FV and bone marrow and spleen cells were harvested at 3 dpi. Cells were isolated and stained for NK cells (CD3−NK1.1+CD49b+, dotted bars) and NKT cells (CD3+NK1.1+, shaded). Absolute numbers of NK1.1+ cells are displayed in a (bone marrow) and b (spleen). The percentages of CD4+ (black bars), CD8+ (gray bars) and double-negative (DN, white bars) subsets of NKT cells in FV-infected mice were displayed in c (bone marrow) and d (spleen). NKT cells were analyzed for the activation markers CD69 (e), CD86 (f) and CD43 (g) using flow cytometry. In e, f and g, mean percentage (±SEM) of bone marrow cells are depicted with white bars whereas splenocytes are displayed in gray bars. A minimum of five mice per group were used. Experiments were repeated at least five times. Statistically significant differences between naïve and FV-infected mice were determined by the Mann–Whitney test and are indicated by single asterisk for p < 0.05 and double asterisk for p < 0.01