Fig. 4
From: HIV integration sites in latently infected cell lines: evidence of ongoing replication
The effect of raltegravir on integration sites and 2-LTR circles using the ACH-2 cell line. The ACH2 cell line was passaged multiple times in the presence (red) and absence (blue) of raltegravir and a the frequency of 2-LTR circles per million cells and b number of unique integration sites were quantified. Positive controls included CEM cells spiked with 2-LTR circles; negative controls were CEM cells, CEM cells spiked with pNL4-3, J-Lat 10.6 and J-Lat15.4. *p = 0.018 and 0.02, **p = 0.004