RNA
|
HIV-1 NC
|
SIV NC
|
HIV-1 Gag∆p6
|
---|
K
d(1M) (M)a
|
Z
beff
|
K
d(1M) (M)a
|
Z
beff
|
K
d(1M) (M)a
|
Z
beff
|
---|
HIV-1 TARpolyA
|
(1.2 ± 1.2) × 10−4
|
2.4 ± 0.4
|
(8.2 ± 2.3) × 10−5
|
2.1 ± 0.1
|
(1.3 ± 0.1) × 10−1
|
8.7 ± 0.5
|
HIV-1 Psi
|
(4.0 ± 2.6) × 10−6
|
1.2 ± 0.1
|
(3.9 ± 1.5) × 10−6
|
1.3 ± 0.1
|
(1.8 ± 2.0) × 10−4
|
5.0 ± 2.0
|
SIV TAR
|
(8.1 ± 6.5) × 10−5
|
2.6 ± 0.4
|
(6.2 ± 4.3) × 10−5
|
2.4 ± 0.3
|
(1.2 ± 1.9) × 10−1
|
8.0 ± 1.4
|
SIV Psi
|
(1.0 ± 0.7) × 10−4
|
3.6 ± 0.9
|
(7.2 ± 3.1) × 10−5
|
3.0 ± 0.3
|
(4.7 ± 4.0) × 10−2
|
8.8 ± 1.0
|
- All values represent the average of at least three trials with the associated standard deviation
-
aKd(1M) is the affinity at 1 M NaCl, which represents the specific, non-electrostatic component of binding
-
bZeff is the number of Na+ ions released upon protein binding, and reflects the number of electrostatic contacts between protein and RNA