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Table 2 Binding parameters determined from FA salt-titration assays

From: Mechanistic differences between HIV-1 and SIV nucleocapsid proteins and cross-species HIV-1 genomic RNA recognition

RNA HIV-1 NC SIV NC HIV-1 Gag∆p6
K d(1M) (M)a Z eff b K d(1M) (M)a Z eff b K d(1M) (M)a Z eff b
HIV-1 TARpolyA (1.2 ± 1.2) × 10−4 2.4 ± 0.4 (8.2 ± 2.3) × 10−5 2.1 ± 0.1 (1.3 ± 0.1) × 10−1 8.7 ± 0.5
HIV-1 Psi (4.0 ± 2.6) × 10−6 1.2 ± 0.1 (3.9 ± 1.5) × 10−6 1.3 ± 0.1 (1.8 ± 2.0) × 10−4 5.0 ± 2.0
SIV TAR (8.1 ± 6.5) × 10−5 2.6 ± 0.4 (6.2 ± 4.3) × 10−5 2.4 ± 0.3 (1.2 ± 1.9) × 10−1 8.0 ± 1.4
SIV Psi (1.0 ± 0.7) × 10−4 3.6 ± 0.9 (7.2 ± 3.1) × 10−5 3.0 ± 0.3 (4.7 ± 4.0) × 10−2 8.8 ± 1.0
  1. All values represent the average of at least three trials with the associated standard deviation
  2. aKd(1M) is the affinity at 1 M NaCl, which represents the specific, non-electrostatic component of binding
  3. bZeff is the number of Na+ ions released upon protein binding, and reflects the number of electrostatic contacts between protein and RNA