Fig. 4

Kinetics of SIV minus-strand transfer in the presence of SIV and HIV-1 NC proteins. Reactions were incubated with the indicated concentrations of SIV or HIV-1 NC for 60 min at 37 °C and were analyzed as described in “Methods” section. a Representative gels showing DNA species present in reactions with 1.25 µM SIV or HIV-1 NC. The transfer product (T) and (−) SSDNA are indicated to the left of the gel image and these were the only two bands that appeared on the gel. Note that self-priming products [18, 19, 100, 114] were not formed under the conditions used for these assays. Lane c shows the migration of (−) SSDNA in the absence of other reactants. b, c The % strand transfer product formed was plotted against time of incubation for reactions with SIV NC (b) or HIV-1 NC (c). Error bars represent the SD from three or more independent experiments