From: Ultrasensitive single-genome sequencing: accurate, targeted, next generation sequencing of HIV-1 RNA
Method | Enzyme | cDNA starting copy | Qualified sequences | % Sequences excludeda | Final # sequences | % Population represented |
---|---|---|---|---|---|---|
LP-PCR-1 | AmpliTaq Goldb | 34,094 | 11,215 | 40 | 6729 | 20 |
uSGS | AmpliTaq Goldb | 34,094 | 11,859 | 7 | 11,060 | 32 |
LP-PCR-1 | Kapa Uracil+b | 34,094 | 3566 | 11 | 3188 | 9 |
uSGS | Kapa Uracil+b | 34,094 | 18,855 | 2 | 18,512 | 54 |
LP-PCR-1 | Platinum Taqc | 14,410 | 2789 | 82 | 558 | 4 |
uSGS | Platinum Taqc | 88,560 | 25,773 | 10 | 22,938 | 26 |