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Table 1 Comparison of cDNA amplification efficiency among methods using the same HIV-1 site specific RT-Primer ID primer

From: Ultrasensitive single-genome sequencing: accurate, targeted, next generation sequencing of HIV-1 RNA

 

uSGS

LP-PCR-1

AVG

STDV

AVG

STDV

A.

 Copies of cDNA by qPCR

134,193

45,940

134,193

45,940

 Total # unique primer IDsa

39,597

7491

17,614

6442

 % cDNA amplified

30%

13%

13%

3.3%

 

uSGS

LP-PCR-2

LP-PCR-3

AVG

STDV

AVG

STDV

AVG

STDV

B.

 Copies of cDNA by qPCR

81,040

21,192

81,040

21,192

81,040

21,192

 Total # unique primer IDsa

25,111

9046

15,794

1392

11,565

482

 % cDNA amplified

31%

4.9%

21%

2.5%

14%

2.2%

  1. All results are taken from an average of 3 separate experimental libraries prepared from each method
  2. aTotal number of consensus sequences above the Zhou algorithm cutoff [19]