Fig. 3
From: Ultrasensitive single-genome sequencing: accurate, targeted, next generation sequencing of HIV-1 RNA
Snapshot of sequence alignments of library construction obtained from clinical sample. Small subsets of supermajority sequence alignments obtained from a donor sample using the (a) LP-PCR or (b) uSGS methods of NGS library construction. Dashes (“–” in red) which have been placed in the consensus sequences by the bioinformatics pipeline in positions with <80% identity in sequences in a given daughter set, which is indicative of recombination during PCR. Asterisks mark positions with diverse bases in the uSGS data where no PCR recombination is seen