Fig. 7

The block to single-cycle infection induced by R848 is not caused by IFN. a Monocytes from 3 healthy donors (one bar per donor) were untreated (mock) or treated with 10 µM R848-treated (blue) and after 24 h, the culture medium was collected and type-I IFN was quantified by bioassay. b Monocytes were incubated with different indicated concentrations of R848. After 24 h, the supernatant was collected and proinflammatory cytokines were quantified by cytokine bead array. c Monocytes from 2 healthy donors (dot and square) treated with 10 µM R848 (blue) or 100 U/mL IFNα with (open symbol) or without (closed symbol) IFN blocking antibody cocktail (±αIFN AB) were infected with Vpx-containing HIV-1 luciferase reporter virus. Luciferase activity was measured 72 h post-infection. **p ≤ 0.01 (d) PBMC were treated for 24 h with 100 U/mL IFNα, 10 μM R848 or 0.1, 1, 10 or 100 ng/mL IL-1β after which the cells were infected with Vpx-containing (HIV1 X+) or lacking (HIV1) HIV-1 luciferase reporter virus. Luciferase activity was measured 72 h post-infection