Skip to main content
Fig. 6 | Retrovirology

Fig. 6

From: TLR7/8 agonist induces a post-entry SAMHD1-independent block to HIV-1 infection of monocytes

Fig. 6

R848 does not prevent the infection of CD4+ T cells and MDDC. a Activated CD4+ T cells from 2 healthy donors were treated with 10 µM R848 (blue) or 100 U/mL IFNα (light grey) or untreated (dark grey) for 24 h and then infected with Vpx-containing (HIV1 X+) or lacking (HIV1) HIV-1 GFP reporter virus or with control supernatant (mock). Infectivity was quantified as percent GFP+ cells 72 h post-infection. b MDDC from 3 healthy donors (one bar per donor) were incubated with 10 µM R848 (blue) or 100 U/mL IFNα for 24 h or untreated (−). The cells were then infected with Vpx-containing (HIV1 X+) HIV1 GFP reporter virus. Uninfected cells (mock) and Nevirapine (NVP) treated cells served as controls. c MDM from 3 healthy donors (one bar per donor) were pretreated for 24 h with 10 µM R848 (blue) or untreated and then infected with 3 × 104 (low) or 3 × 105 (high) cps of Vpx-containing HIV1 luciferase reporter virus. Luciferase activity was measured 72 h post-infection. *p ≤ 0.05, **p ≤ 0.01 and ***p ≤ 0.001

Back to article page