TY - JOUR AU - Hofmann, Henning AU - Vanwalscappel, Bénédicte AU - Bloch, Nicolin AU - Landau, Nathaniel R. PY - 2016 DA - 2016/12/01 TI - TLR7/8 agonist induces a post-entry SAMHD1-independent block to HIV-1 infection of monocytes JO - Retrovirology SP - 83 VL - 13 IS - 1 AB - Monocytes, the primary myeloid cell-type in peripheral blood, are resistant to HIV-1 infection as a result of the lentiviral restriction factor SAMHD1. Toll-like receptors recognize microbial pathogen components, inducing the expression of antiviral host proteins and proinflammatory cytokines. TLR agonists that mimic microbial ligands have been found to have activity against HIV-1 in macrophages. The induction of restriction factors in monocytes by TLR agonist activation has not been well studied. To analyze restriction factor induction by TLR activation in monocytes, we used the imidazoquinoline TLR7/8 agonist R848 and infected with HIV-1 reporter virus that contained packaged viral accessory protein Vpx, which allows the virus to escape SAMHD1-mediated restriction.  SN - 1742-4690 UR - https://doi.org/10.1186/s12977-016-0316-3 DO - 10.1186/s12977-016-0316-3 ID - Hofmann2016 ER -