Fig. 1
From: Antibody-dependent CD56+ T cell responses are functionally impaired in long-term HIV-1 infection
Degranulation of CD56+ T cells could be triggered by CD16 cross-linking. a Gating strategies of CD56+ T, CD56+ NK, and CD56− T cells from lymphocytes in a representative subject. The percentages of CD16+ and CD32+ cells in different subsets were also shown. b Frequencies of CD16+ and CD32+ cells in CD56+ T, CD56+ NK, and CD56− T cells of healthy donors (n = 10). Data were shown as mean ± SD. c Images of CD3, CD56, CD16, and CD32 expression on CD56+ T, CD56+ NK, and CD56− T cells at single-cell level. Data was performed on an ImageStreamχ MarkII system. Images of three separate samples were performed with similar results. d Representative flow plots depicted the ability of purified CD56+ T cells to degranulate in response to CD16/CD32 cross-linking with anti-CD16, anti-CD32, or nonspecific isotype antibodies. Positive responses were reflected by shifted CD107a expression. e The frequencies of CD107a+ CD56+ T cells upon CD16 or CD32 cross-linking in healthy donors (n = 10). The horizontal bar indicated median values. All P values were two-tailed and considered significant when less than 0.05