Membrane bound modified form of clade B Env, JRCSF is suitable for immunogen design as it is efficiently cleaved and displays all the broadly neutralizing epitopes including V2 and C2 domain-dependent conformational epitopes

Table 1 Representative neutralization data of JRFL and JRCSF pseudotyped viruses [3, 34, 38]

mAb epitope	mAb	JRFL	JRCSF
CD4-bs	b12	+++	++
	VRC01	+++	++
	NIH45-46	++++	+++
	CH103	++++	+++
	3BNC117	++++	+++
	8ANC131	+++	++
CD4i	17b	−	−
V1V2V3 glycan-dependent	PG9	−	++++
	PG16	−	++++
	PGT145	+	++++
	PGT121	+++	+++
	PGT128	++++	++++
	2G12	+	++
MPER	10E8	++	++
	4E10	+	+
	2F5	+	+
FP	VRC34	+	±

ISSN: 1742-4690