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Fig. 4 | Retrovirology

Fig. 4

From: Membrane bound modified form of clade B Env, JRCSF is suitable for immunogen design as it is efficiently cleaved and displays all the broadly neutralizing epitopes including V2 and C2 domain-dependent conformational epitopes

Fig. 4

The entire V2 region of JRCSF is the primary determinant of JRCSF specificity towards PG9/PGT145 class of bNAbs. a Schematic representation showing swapping mutants of JRFL domains in JRCSF used in this experiment. b Sequence analysis of V2 domain of JRFL and JRCSF and position of mutants used in this experiment. c FACS-based cell surface staining assay of JRFL, JRCSF and the domain swapped mutants V1*C2 and V2*C2 with PG9 and PGT145 over a range of antibody concentrations. d Comparative binding of V2 domain mutants of JRCSF in a FACS-based cell surface assay with PG9 and PGT145 over a range of antibody concentrations. The bar diagram represents the binding of the respective antibodies to the wild type and different mutant Envs at 20 µg/ml of antibody concentration

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Retrovirology

ISSN: 1742-4690

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