Fig. 2

Detection of Tax, miR21, miR155 and VEGF in ATL-derived exosomes. a Histogram representing the reciprocal value of Cq at which Tax expression was detected in leukemic cells and their derived exosomes (***p ≤ 0.005). b A representative western blot showing Tax protein expression in leukemic cell lysates (100 µg) and their derived exosomes (15 µg). GAPDH was used as an internal loading control for the cell lysates. c Histogram representing the reciprocal value of Cq at which Tax expression was detected in p-ATL cells and their exosomes, as compared to HuT-102 cells. d Histogram representing the normalized expression of miR-155 and miR-21 in HuT-102 cells and their derived-exosomes, as detected by qPCR (*p ≤ 0.05; **p ≤ 0.01). e Histogram representing VEGF protein levels detected in the supernatants or exosomes isolated from HuT-102 cells by ELISA