Fig. 2

TRIM11 accelerates HIV-1 uncoating during infection. a, b HEK293 cells stably expressing TRIM11-HA and pCDH were infected with 50 ng/ml (p24^gag) HIV-1 and viral transduction was assessed at 24 h post infection by luciferase activity (a) and late reverse transcription levels were assessed at 3 h post infection by qPCR (b). c HEK293 cells transfected with pCMV-myc-TRIM11 or empty vector were infected with HIV-1 with or without VSV-G envelop at 4 °C for 30 min and then incubated at 37 °C for the indicated time. The cell lysates were centrifuged through 50 % sucrose cushion, and the pellet was resuspended in SDS sample buffer. The input and pellet were analyzed by Western blotting with an anti-myc and an anti-p24 antibody. The levels of p24 in pelletable and input fractions were measured by densitometry and pellet/input ratio was calculated. d–f Similar experiments were carried out as described in a–c, with HEK293 cells in which TRIM11 had been stably knocked down with shRNA. Error bars represent the standard deviations from three independent replicates of the same experiments. *P < 0.05