Fig. 5

Responsiveness to poly(I:C) was defective in U1 cells, when compared to U937 cells. U937 and U1 cells were transfected (Lipofectamine^®2000) with media alone or 0.1, 1, or 10ug/mL of poly(I:C) for 48 h. a Minimal constitutive secretion of of IFNα/β (n = 5) was observed in U937 and U1 cells, as quantified using using the HEK-Blue™ IFNα/β biologic assay (dashed line denotes lower limit of detection of assay = 0.15 U/mL). b Secretion of IFNα/β by U937 cells, but not latently HIV-infected U1 cells was detected following poly(I:C) transfection (n = 5). c Poly(I:C) induced ISG15 expression (n = 5), as measured by flow cytometry, was observed only in the HIV-uninfected U937 cells. d Similarly, poly(I:C) induced expression of PKR (n = 5) was impaired in U1 cells, but not in U937 cells. Representative histogram and cumulative summary of poly(I:C)-induced ISG expression is shown. ^† p = 0.03; ^‡ p = 0.0004 as measured by one-way ANOVA and p < 0.05 by pairwise Dunnett’s Test compared to unstimulated cells. *p < 0.01; **p < 0.001, ***p < 0.05 by Two-way ANOVA with Bonferroni post-test for multiple comparisons