Fig. 5

Transmitter and recipient viruses show comparable entry kinetics. a Scheme of time-of-inhibitor addition experiment on TZM-bl cells. b Entry kinetic curves for one representative transmission pair with two transmitter Env-pseudoviruses depicted in red and the recipient Env-pseudovirus in blue. The relative infectivity reached after 120 min was set as 100 % and data points were normalized to this value. Data points are mean and standard deviation from three independent experiments each performed in duplicates. c Time to 50 % entry was determined out of entry kinetic curves and median time to 50 % entry of transmitter Env-pseudoviruses was compared to recipient Env-pseudoviruses applying a Wilcoxon matched-pairs signed rank test. d Linear regression analysis of T-20 IC₅₀ and time to 50 % entry with r² and p value depicted. Data for transmitters and recipients are indicated in red and blue, respectively