Table 2 A. Fold changes in neutralization sensitivity of the Env-pseudotyped viruses to INDO-SA 2007 plasma depleted with the monomeric (4-2.J41) gp120 and trimeric (BG505-SOSIP.664) gp140 proteins. B. Specificity of INDO-SA 2007 BCN plasma antibodies to known epitopes in variable loops. C. Presence of MPER directed neutralizing antibodies in INDO-SA 2007 plasma
A | Fold decrease in ID50 a | |
|---|---|---|
Monomer (4-2.J41 gp120) | Trimer (BG505-SOSIP.664) | |
25711-2.4 | >10.00 | >20.00 |
4-2.J41 | 3.00 | 5.37 |
7.J20 | 7.06 | >14.00 |
Du172.17 | >7.00 | 7.14 |
CAP84.32 | >7.00 | >11.00 |
CAP239.G3 | 6.00 | 5.91 |
B | |||
|---|---|---|---|
HIV-1 Env mutants | Region | Neutralization titer (ID50) | Fold decrease in ID50 b |
HIV-1 25711-2.4 | Wild type | 1822.04 | – |
HIV-1 CAP239.G3 | Wild type | 1096.00 | – |
HIV-1 25711-2.4 N160A | V2 | 1484.35 | 1.22 |
HIV-1 CAP239.G3 N160A | V2 | 4184.00 | 0.26 |
HIV-1 25711-2.4 R166A | V2 | 1833.00 | 0.99 |
HIV-1 25711-2.4 K169E | V2 | 2587.29 | 0.70 |
HIV-1 25711-2.4 N301A | V3 stem | 749.81 | 2.42 |
HIV-1 25711-2.4 N332A | V3 stem | 357.36 | 5.09 |
HIV-1 CAP239.G3 N332A | V3 stem | 204.60 | 5.35 |
C | ||
|---|---|---|
HIV-2/HIV-1 chimera | Region of HIV-1c | ID50 |
HIV-2 7312A | HIV-2 wild type | <20 |
HIV-2 7312A-C1C | Clade C MPER (ELLALDKWASLWNWFDITKWLWYIK) | 63.77 |
HIV-2 7312A-C4 | 4E10 epitope (NWFDITKWLWYIK) | <20 |
HIV-2 7312A-C6 | 4E10 minimal (NWFDIT) | <20 |