Fig. 2
APOL1 polymorphism regulates HIV-1 persistence in CIHPs. a Statistical histogram graph showing HIV-1 accumulation in CIHPs transiently transfected with a APOL1-WT expressing vector compared to the control vector (Ctrl) following a time course incubation (3 and 6 h) with the virus. Results are expressed as relative fold change of HIV-1 DNA RU5 in APOL1-WT transfected cells compared to Ctrl and normalized to GAPDH gene (N = 4). b Time-course experiments showing the HIV-1 accumulation in CIHPs stable transfected with the control vector (Ctrl) or the specific APOL1-WT or APOL1-G1/G2 expressing vectors. Results are expressed as relative fold change of HIV-1 DNA RU5 in HIV-1 incubated CIHPs versus non HIV-1 treated cells and normalized to GAPDH gene (N = 4). c Rescue of infectious HIV-1 by CD4pos T lymphocytes co-cultured in time-course experiments (1, 3, 5 and 7 days) with HIV-1 pulsed CIHPs stable transfected with APOL1-WT or APOL1-G1 or APOL1-G2 compared with cells transfected with control vector (Ctrl). Since HIV-1 internalization in human podocytes is characterized by an abortive HIV-1 infection, cell lysates were collected and analyzed by qPCR for HIV-1 Gag gene not present in podocytes and indicative of a productive infection in CD4pos T cells. Results are expressed as fold increased of HIV-1 DNA Gag gene in PBMC co-cultured with HIV-1-pulsed CIHPs transfected with APOL1-WT or APOL1-G1 or APOL1-G2 or an empty vector (Ctrl) compared to their HIV-1 untreated counterparts and normalized to the amount of GAPDH gene (N = 4). d Statistical histogram graph showing TFEB gene expression in CIHPs transiently transfected with control vector (Ctrl) or APOL1-WT or APOL1-G1 or APOL1-G2 expressing vector. Results are expressed as fold change of transfected cells compared to Ctrl and normalized to the expression of GAPDH gene (N = 4). P values: *<0.05, **<0.01; ***<0.001