Fig. 8

CCR6⁺DN and CCR6⁺DP subsets are permissive to HIV infection in vitro. a PBMCs from healthy individuals were stained with a cocktail of fluorochrome-conjugated CD3, CD4, CD45RA, CCR4, CXCR3, CCR6, and CCR5 or CXCR4 Abs. The gating strategy for the identification of distinct CCR6⁺ and CCR6⁻ T-cell subsets was designed as in Fig. 1a. The frequency of cells expressing CCR5 (left panel) and CXCR4 (right panel) was analyzed within the Th17, Th1Th17, CCR6⁺DN and CCR6⁺DP subsets. Paired t-Test p-values are indicated on the figures. Horizontal bars indicate median values. b Memory CCR6⁺ subsets from three HIV-uninfected subjects were sorted and stimulated via CD3/CD28 for 4 days, as in Fig. 1e. Cells were exposed to a highly infectious R5 strain HIV-ADA8. Levels of HIV-p24 were quantified by ELISA in cell supernatants at day 3 posy-infection