Fig. 2

CCR6⁺DN and CCR6⁺DP cells express unique transcriptional signatures. Genome-wide transcriptional profiling was performed on sorted matched Th17, CCR6⁺DN, and CCR6⁺DP (n = 4–6) isolated from the peripheral blood of HIV-uninfected individuals, as in Fig. 1f. Shown are (a) volcano representation of differentially expressed probe sets in CCR6⁺DN versus Th17, CCR6⁺DP versus Th17, and CCR6⁺DN versus CCR6⁺DP (depicted in red: p values <0.05 and fold change cut-off 1.3). b, d For the same contrasts, shown are heat maps depicting top modulated pathways identified using Ingenuity Pathway Analysis (IPA) (b) and Gene Set Variation Analysis (GSVA) canonical pathways (c) and biological functions (d). Genes up and down regulated in different subsets are represented in red and blue, respectively. e Expression of STAT3, BCL6, LEF1, and TERC mRNA was quantified by real-time RT-PCR (mean ± SEM; n = 3–4). Paired t-Test p-values are indicated on the figures