Fig. 6
From: HIV-1 capsid uncoating initiates after the first strand transfer of reverse transcription
Modelling HIV-1 uncoating relative to reverse transcription. For each panel, points represent experimental data and solid lines indicate model solutions. a, b 293T cells were infected with GFP-HIV VLP in the presence of 10 μM NVP for 4 h starting at 0, 1 or 2 h.p.i. Early (a) and late (b) cDNA was analysed by qPCR. c, d CsA washout assays. OMK cells were infected with GFP-HIV VLP in the presence of c 10 μM NVP for 4 h starting at 0, 1 or 2 h.p.i. or d increasing concentrations of NVP for 0–2 h. CsA was removed from each sample at the indicated time. After 72 h the percentage of GFP positive cells was measured and plotted relative to the percentage of GFP positive cells following infection in the absence of NVP when CsA was removed 7 h.p.i. Each panel shows the mean and SD of at least 3 independent experiments. Model solutions were computed using Eq. (2) stated in the text and the best fit parameters \( k_{1} = 0.29\,{\text{h}}^{ - 1} \); \( t_{bp} = 2.4 \times 10^{ - 4} \,{\text{h}} \); \( k_{deg} = 0.3\,{\text{h}}^{ - 1} \); \( bp^{*} = 974 \) bases. Other parameters were: K NVP  = 0.1 μM, Ninit = 100, bp–sscDNA = 137; bp(+)strand = 3958