Fig. 1

Effect of NVP on cDNA synthesis and uncoating. a CsA washout assay. OMK cells were infected with GFP-HIV VLP in the presence of CsA and increasing concentrations of NVP. CsA was removed from each sample at the indicated time and NVP was removed from all samples 2 h.p.i. After 72 h, the percentage of GFP positive cells was measured for each sample and plotted relative to the percentage of GFP positive cells following infection in the absence of NVP when CsA was removed 4 h.p.i. b, c 293T cells were infected with GFP-HIV VLP in the presence of increasing concentrations of NVP. NVP was removed from the media after 2 h. At the indicated times post-infection, the level of cDNA corresponding to a early (−sscDNA) or b late ((+)strand) reverse transcription products were analysed by qPCR. d CsA washout assay as in (a), except that 10 μM NVP was added for 4 h starting at 0, 1 or 2 h.p.i. The percentage of GFP positive cells for each sample was plotted relative to the percentage of GFP positive cells following infection in the absence of NVP when CsA was removed 7 h.p.i. e, f 293T cells were infected with GFP-HIV VLP in the presence of 10 μM NVP for 4 h starting at 0, 1 or 2 hp.i. Early (e) and late (f) cDNA was analysed by qPCR. Each panel shows the mean and SEM of at least 3 independent experiments