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Fig. 9 | Retrovirology

Fig. 9

From: Mutagenesis of N-terminal residues of feline foamy virus Gag reveals entirely distinct functions during capsid formation, particle assembly, Gag processing and budding

Fig. 9

Pol is not incorporated into myr-Gag capsids. a Construction of FFV proviral Src-Gag mutants in the pCF-7 provirus. A myr signal derived from the human cellular protein Src was added onto the N-termini of FFV wt Gag and Gag mutants mLQQLY, mHGDII and mPGDRW (shown in Italic and bold). b 293T cells were transfected in 10 cm dishes with 8 µg of proviral pCF-7-based wt Gag (lane 1), Gag mutants (mLQQLY, mHGDII, mPGDRW, Src-Gag, Src-mLQQLY, Src-mHGDII and Src-mPGDRW) (lanes 2–8), or pcDNA (lane 10). Cell lysates and VLPs in supernatants were analyzed by SDS-PAGE. Positions of Gag p48 and p52, Pol precursor p127Pol and PR-RT-RN domain p65Pol are marked. c Intracellular capsid assembly of FFV virus, Gag sub-viral particles (SVPs) and Src-Gag SVPs were visualized by transmission electron microscopy of transfected and fixed 293T cells. Transmission electron micrographs show representative thin sections of the cells. Scale bars are 500 nm in length

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