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Fig. 6 | Retrovirology

Fig. 6

From: Mutagenesis of N-terminal residues of feline foamy virus Gag reveals entirely distinct functions during capsid formation, particle assembly, Gag processing and budding

Fig. 6

Subcellular localization of wt and single-mutant FFV Gag proteins. HeLa cells were transfected with proviral pCF-7-based Gag mutants (R32A, G36A, G39A and R43A) and the parental provirus pCF-7. pcDNA plasmid was used for transfection as negative control. At 36 h p.t., cells were fixed and stained with a rabbit polyclonal antiserum generated against FFV Gag and Alexa-488-conjugated secondary antibody. Nuclei were stained with DAPI. Scale bars represent 50 µm

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