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Fig. 5 | Retrovirology

Fig. 5

From: Mutagenesis of N-terminal residues of feline foamy virus Gag reveals entirely distinct functions during capsid formation, particle assembly, Gag processing and budding

Fig. 5

Gradient analysis of intracellular mutant Gag capsids. 293T cells were transfected in 10 cm dishes with 8 µg of pCF-7-Q11A, H25A, R32A, G36A, W38A, G39A, R43A, L51A, D53A or wild-type pCF-7. Two days p.t., cytoplasmic extracts of transfected 293T cells were prepared and used for sucrose gradient sedimentation. Each, six fractions (S1–S6, 700 μl) were collected from the top of the gradient and analyzed by immunoblotting. Positions of FFV Gag p48 and p52 are marked

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