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Fig. 10 | Retrovirology

Fig. 10

From: Mutagenesis of N-terminal residues of feline foamy virus Gag reveals entirely distinct functions during capsid formation, particle assembly, Gag processing and budding

Fig. 10

Myristoylated Gag interferes with Gag processing and viral titer in a trans-dominant negative fashion. a 293T cells were co-transfected with different ratios of the FFV wt genome pCF-7 and the proviral Src Gag clone (lanes 1–6) or the empty vector pcDNA (lanes 1′–6′). Cell lysates and VLPs in supernatants were analyzed by SDS-PAGE. FFV Gag p48 and p52 are marked. b Two days p.t., infectivity was determined by titration of culture supernatants on FeFab cells. Mean titers of three independent experiments are given. Error bars represent the standard deviation

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