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Fig. 1 | Retrovirology

Fig. 1

From: Mutagenesis of N-terminal residues of feline foamy virus Gag reveals entirely distinct functions during capsid formation, particle assembly, Gag processing and budding

Fig. 1

Phenotype of N-terminal Gag deletion mutants. a Schematic of the FFV N-terminal Gag deletion mutants. Four Gag mutants (E4AΔ5, E4AΔ7, E4AΔ9, E4AΔ11) were constructed based on the proviral clone pCF-7. b 293T cells were transfected in 10 cm dishes with 8 µg of proviral pCF-7-based Gag mutants (lanes 1–4), the wild-type parental pCF-7 (lane 5) or pcDNA (lane 6). Aliquots of cell lysates and VLPs in supernatants were analyzed by SDS-PAGE. Positions of Gag p48 and p52, full-length Env precursor gp130Env, mature processed Env gp48™, Pol precursor p127Pol and PR-RT-RN domain p65Pol are marked

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