Table 1 PCR primer pairs used for amplification of overlapping subgenomic PTLV-1 fragments
From: Development of neurologic diseases in a patient with primate T lymphotropic virus type 1 (PTLV-1)
Fragment no. | Name | Primer sequences 5′–3′ |
|---|---|---|
1 | Px 23 ACDFN | Fwd: TCATTTCTACTCTCACA |
| Â | LTR U5E | Rev: CGCAGTTCAGGAGGCACCACAGGCG |
2 | LTR400-F | Fwd: CATCCACGCCGGTTGAGTCGC |
| Â | Gag1400-R | Rev: GCTGGTGATGGAGGGAAGCTA |
3 | Gag1350-F | Fwd: CAAAGACCTCCAAGACCTCCT |
| Â | Pol2520-R | Rev: TCTAGCCCAAGGACGGCTGGC |
4 | Pol AG 1 | Fwd: GTCGTGATGCCTTACAACAATGCC |
| Â | Pol AG 2 | Rev: GGGCATGTAGCCAGACAAGTGGCC |
5 | SK54 | Fwd: CTTCACAGTCTCTACTGTGC |
| Â | SK111 | Rev: GTGGTGAAGCTGCCATCGGGTTTT |
6 | SK110 | Fwd: CCCTACAATCCAACCAGCTCAG |
| Â | SG453 | Rev: GCGGGATCCTAGGGTGGGAACAG |
7 | ENV 1 | Fwd: TCAAGCTATAGTCTCCTCCCCCTG |
| Â | ENV 2 | Rev: GGGAGGTGTCGTAGCTGACGGAGG |
8 | Env3-F | Fwd: ACAAACTGGAATCACCCTTGTTGC |
| Â | SK44 | Rev: GAGCCGATAACGCGTCCATCG |
9 | SK43 | Fwd: CGGATACCCAGTCTACGTGT |
| Â | Tax ddPCR-R | Rev: TGAGGCCGTGTGAGAGTAGA |