Fig. 2

Interaction of HIV-1 Gag and RPL7 in infected cells and virion incorporation of RPL7. a Interaction of HIV-1 Gag and RPL7 in infected cell. Cell lysates of naïve CEM-SS (Lanes 1 and 4) or of HIV-1 LAI infected CEM-SS (lanes 2 and 4) were subjected to IPs 3 days post infection using anti-p24 antibody and protein A beads. The IP was followed by western blotting with anti-p24 and anti-RPL7 antibodies revealed by protein A-HRP and anti-rabbit HRP conjugate, respectively. Additionally, the total amount of protein loaded on the gel was controlled by anti-GAPDH antibody (input, lanes 1 and 2). A bead control using the infected cell lysate in the absence of p24 antibody was also performed (lane 3), confirming the specific binding of Gag and its processed forms (p24, p41, p55). b RPL7 is incorporated into HIV-1 particles. HIV-1 BaL virus stock produced on PHA-activated PBMC were purified and concentrated before been analyzed by western blotting using anti-p24 and anti-RPL7 antibodies. The control represents the purified supernatant from mock-infected PBMC using the same purification protocol