Fig. 8

Properties of the defective B 202G Env at the surface of the viral particles. a Viral titer (ng of p24) estimated after retention of viral supernatant on ELISA plates coated with the antibody D7324. b Western blot on purified viral particles using antibody D7324. c Viral titer (ng of p24) estimated after retention of viral supernatant on ELISA plates coated with the antibody PGT 145. d, e Measure of the stability of the gp120 protein at the surface of the viral particle. d Western blot performed on purified virions carrying either wild type B or B 202G Env proteins as function of the incubation time (indicated, in hours, above each lane) at 37 °C. The identity of the different proteins has been inferred by their molecular mass and is indicated with arrows. The gel shows a representative result of three independent experiments. e The bands corresponding to gp120 and to gp41 in three western blots (as the one shown in d) have been quantified as indicated in “Methods” section and the corresponding values are expressed as gp120/gp41 ratio as function of the incubation time. Triangles wt B Env; squares B 202G Env