The effect of HIV-1 Vif polymorphisms on A3G anti-viral activity in an in vivo mouse model

Retrovirology

Table 2 Mutation frequencies in env of F-MLV-Vif proviruses bearing different Vif variants in splenic DNA of infected APOBEC3 KO, A3G^high and A3G^low transgenic mice

	FMLV-2A			NL43			W11R			K22E
	KO	A3G^Low	A3G^High	KO	A3G^Low	A3G^High	KO	A3G^Low	A3G^High	KO	A3G^Low	A3G^High
#G to A	7	27	116	1	2	12	1	1	18	5	57	63
#other	37	19	43	67	28	71	13	6	12	26	34	49
#mice	3	3	3	3	3	4	3	4	3	3	3	3
#clones	42	40	41	42	41	53	49	42	45	50	45	48
#bp	24,612	23,440	24,026	24,612	24,026	31,058	28,714	22,268	26,370	29,300	26,370	28,128
G to A freq. ×10⁻³	0.28	1.15	4.83	0.04	0.08	0.39	0.03	0.04	0.68	0.17	2.16	2.24
Other freq. ×10⁻³	1.50	0.81	1.79	2.72	1.17	2.29	0.45	0.27	0.46	0.89	1.29	1.74
#stop codons	2	4	46	0	1	4	0	0	7	0	15	31

Analysis was performed on DNA cloned from a subset of the infected mice in Fig. 2 (3–4 mice/group; 10–15 sequences/mouse). F-MLV has a total of 58 GG motifs in the 586 bp region that was sequenced

ISSN: 1742-4690