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Table 2 Mutation frequencies in env of F-MLV-Vif proviruses bearing different Vif variants in splenic DNA of infected APOBEC3 KO, A3Ghigh and A3Glow transgenic mice

From: The effect of HIV-1 Vif polymorphisms on A3G anti-viral activity in an in vivo mouse model

  FMLV-2A NL43 W11R K22E
KO A3GLow A3GHigh KO A3GLow A3GHigh KO A3GLow A3GHigh KO A3GLow A3GHigh
#G to A 7 27 116 1 2 12 1 1 18 5 57 63
#other 37 19 43 67 28 71 13 6 12 26 34 49
#mice 3 3 3 3 3 4 3 4 3 3 3 3
#clones 42 40 41 42 41 53 49 42 45 50 45 48
#bp 24,612 23,440 24,026 24,612 24,026 31,058 28,714 22,268 26,370 29,300 26,370 28,128
G to A freq. ×10−3 0.28 1.15 4.83 0.04 0.08 0.39 0.03 0.04 0.68 0.17 2.16 2.24
Other freq. ×10−3 1.50 0.81 1.79 2.72 1.17 2.29 0.45 0.27 0.46 0.89 1.29 1.74
#stop codons 2 4 46 0 1 4 0 0 7 0 15 31
  K22H Y40H G143R
KO A3GLow A3GHigh KO A3GLow A3GHigh KO A3GLow A3GHigh
#G to A 3 29 62 0 10 27 2 7 9
#other 22 28 49 18 21 5 11 13 10
#mice 3 3 3 3 4 3 4 3 3
#clones 44 45 46 45 52 41 41 41 39
#bp 25,784 26,370 26,956 26,370 30,472 24,026 24,026 24,026 22,854
G to A freq. ×10−3 0.16 1.10 2.30 0.00 0.33 1.12 0.08 0.29 0.39
Other freq. ×10−3 0.85 1.06 1.82 0.68 0.69 0.21 0.46 0.54 0.44
#stop codons 0 14 25 0 0 7 1 1 1
  1. Analysis was performed on DNA cloned from a subset of the infected mice in Fig. 2 (3–4 mice/group; 10–15 sequences/mouse). F-MLV has a total of 58 GG motifs in the 586 bp region that was sequenced