Table 1 Mutation frequencies and reversion analysis of vif of F-MLV-Vif proviruses bearing different Vif variants in splenic DNA of infected APOBEC3 KO, A3Ghigh and A3Glow transgenic mice
From: The effect of HIV-1 Vif polymorphisms on A3G anti-viral activity in an in vivo mouse model
NL43 | W11R | K22E | K22H | Y40H | G143R | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
KO | A3GLow | A3GHigh | KO | A3GLow | A3GHigh | KO | A3GLow | A3GHigh | KO | A3GLow | A3GHigh | KO | A3GLow | A3GHigh | KO | A3GLow | A3GHigh | |
#G to A | 0 | 2 | 4 | 2 | 1 | 6 | 1 | 14 | 19 | 1 | 10 | 22 | 0 | 5 | 12 | 2 | 2 | 3 |
#other | 8 | 7 | 10 | 9 | 8 | 9 | 9 | 9 | 12 | 7 | 9 | 11 | 8 | 10 | 10 | 9 | 8 | 9 |
#clones | 15 | 14 | 14 | 15 | 14 | 15 | 15 | 12 | 15 | 13 | 14 | 14 | 14 | 14 | 14 | 15 | 15 | 14 |
#bp | 8700 | 8120 | 8120 | 8700 | 8120 | 8700 | 8700 | 6960 | 8700 | 7540 | 8120 | 8120 | 8120 | 8120 | 8120 | 8700 | 8700 | 8120 |
G to A freq. ×10−3 | 0.00 | 0.25 | 0.49 | 0.23 | 0.12 | 0.69 | 0.11 | 2.01 | 2.18 | 0.13 | 1.23 | 2.71 | 0.00 | 0.62 | 1.48 | 0.23 | 0.23 | 0.37 |
Other freq. ×10−3 | 0.92 | 0.86 | 1.23 | 1.03 | 0.99 | 1.03 | 1.03 | 1.29 | 1.38 | 0.93 | 1.11 | 1.35 | 0.99 | 1.23 | 1.23 | 1.03 | 0.92 | 1.11 |
#stop codons | 0 | 0 | 1 | 0 | 1 | 3 | 1 | 4 | 3 | 2 | 5 | 8 | 0 | 1 | 3 | 1 | 1 | 3 |
#revertants | NA | NA | NA | 0/15 | 0/14 | 0/15 | 0/15 | 1/12 | 3/15 | 0/13 | 1/14 | 2/14 | 0/14 | 0/14 | 2/14 | 0/15 | 0/15 | 0/15 |