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Table 3 Restriction activity of selected Fv1 alleles against different retroviruses at different Fv1 expression levels

From: Expression levels of Fv1: effects on retroviral restriction specificities

Vector TYIx-Fv1 LxIY-Fv1
Dox (ng/mL) 0 10 100 1000 0
Fv1b      
 N-MLV 0.46 ± 0.02 0 .21 ± 0.01 0.14 ± 0.02 0.13 ± 0.02 0.09 ± 0.01
 B-MLV 1.09 ± 0.05 0.96 ± 0.05 0.83 ± 0.05 0.77 ± 0.03 0.48 ± 0.02
 NB-MLV 0.93 ± 0.06 0.51 ± 0.03 0.35 ± 0.01 0.33 ± 0.04 0.16 ± 0.01
 EIAV n.d. n.d. n.d. n.d. 0.52 ± 0.01
 FFV n.d. n.d. n.d. n.d. 1.00 ± 0.02
Fv1MIN2      
 N-MLV 0.43 ± 0.02 0.18 ± 0.02 0.14 ± 0.02 0.11 ± 0.02 0.04 ± 0.01
 B-MLV 0.33 ± 0.01 0.16 ± 0.03 0.12 ± 0.01 0.11 ± 0.02 0.04 ± 0.01
 NB-MLV 1.02 ± 0.04 0.52 ± 0.02 0.27 ± 0.03 0.23 ± 0.05 0.10 ± 0.01
 EIAV n.d. n.d. n.d. n.d. 1.08 ± 0.04
 FFV n.d. n.d. n.d. n.d. 1.00 ± 0.02
Fv1SPR1      
 N-MLV 0.70 ± 0.04 0.28 ± 0.01 0.17 ± 0.02 0.16 ± 0.02 0.12 ± 0.01
 B-MLV 0.75 ± 0.04 0.31 ± 0.05 0.19 ± 0.01 0.19 ± 0.02 0.13 ± 0.01
 NB-MLV n.d. n.d. n.d. n.d. 1.17 ± 0.01
 EIAV 0.65 ± 0.06 0.32 ± 0.03 0.24 ± 0.02 0.24 ± 0.02 0.20 ± 0.01
 FFV n.d. n.d. n.d. n.d. 1.02 ± 0.01
Fv1MAC      
 N-MLV 1.10 ± 0.04 0.70 ± 0.02 0.35 ± 0.02 0.30 ± 0.02 0.18 ± 0.01
 B-MLV 1.08 ± 0.09 1.08 ± 0.02 1.10 ± 0.06 1.02 ± 0.02 1.24 ± 0.02
 NB-MLV n.d. n.d. n.d. n.d. 1.24 ± 0.01
 EIAV 0.79 ± 0.02 0.44 ± 0.02 0.27 ± 0.02 0.26 ± 0.03 0.21 ± 0.01
 FFV n.d. n.d. n.d. n.d. 1.01 ± 0.03
Fv1CAR1      
 N-MLV n.d. n.d. n.d. n.d. 1.21 ± 0.07
 B-MLV n.d. n.d. n.d. n.d. 1.15 ± 0.03
 NB-MLV n.d. n.d. n.d. n.d. 1.17 ± 0.01
 EIAV n.d. n.d. n.d. n.d. 1.17 ± 0.09
 FFV 0.95 ± 0.01 0.40 ± 0.04 0.18 ± 0.02 0.18 ± 0.03 0.13 ± 0.01
  1. We arbitrarily divide restriction activity into three levels; Full, shown in bolditalic (restriction ratio ≤0.3), Partial, indicated in italics (0.3 < ratio ≤ 0.7) and None (ratio > 0.7)
  2. Table shows the results from this study using the TYIx-Fv1 inducible vector, with mean and standard deviation values calculated from two independent duplicate experiments. The last column shows results from our previous study using the non-inducible LxIY-Fv1 vector in MDTF cells [8]. n.d., not determined