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Table 3 Restriction activity of selected Fv1 alleles against different retroviruses at different Fv1 expression levels

From: Expression levels of Fv1: effects on retroviral restriction specificities

Vector

TYIx-Fv1

LxIY-Fv1

Dox (ng/mL)

0

10

100

1000

0

Fv1b

     

 N-MLV

0.46 ± 0.02

0 .21 ± 0.01

0.14 ± 0.02

0.13 ± 0.02

0.09 ± 0.01

 B-MLV

1.09 ± 0.05

0.96 ± 0.05

0.83 ± 0.05

0.77 ± 0.03

0.48 ± 0.02

 NB-MLV

0.93 ± 0.06

0.51 ± 0.03

0.35 ± 0.01

0.33 ± 0.04

0.16 ± 0.01

 EIAV

n.d.

n.d.

n.d.

n.d.

0.52 ± 0.01

 FFV

n.d.

n.d.

n.d.

n.d.

1.00 ± 0.02

Fv1MIN2

     

 N-MLV

0.43 ± 0.02

0.18 ± 0.02

0.14 ± 0.02

0.11 ± 0.02

0.04 ± 0.01

 B-MLV

0.33 ± 0.01

0.16 ± 0.03

0.12 ± 0.01

0.11 ± 0.02

0.04 ± 0.01

 NB-MLV

1.02 ± 0.04

0.52 ± 0.02

0.27 ± 0.03

0.23 ± 0.05

0.10 ± 0.01

 EIAV

n.d.

n.d.

n.d.

n.d.

1.08 ± 0.04

 FFV

n.d.

n.d.

n.d.

n.d.

1.00 ± 0.02

Fv1SPR1

     

 N-MLV

0.70 ± 0.04

0.28 ± 0.01

0.17 ± 0.02

0.16 ± 0.02

0.12 ± 0.01

 B-MLV

0.75 ± 0.04

0.31 ± 0.05

0.19 ± 0.01

0.19 ± 0.02

0.13 ± 0.01

 NB-MLV

n.d.

n.d.

n.d.

n.d.

1.17 ± 0.01

 EIAV

0.65 ± 0.06

0.32 ± 0.03

0.24 ± 0.02

0.24 ± 0.02

0.20 ± 0.01

 FFV

n.d.

n.d.

n.d.

n.d.

1.02 ± 0.01

Fv1MAC

     

 N-MLV

1.10 ± 0.04

0.70 ± 0.02

0.35 ± 0.02

0.30 ± 0.02

0.18 ± 0.01

 B-MLV

1.08 ± 0.09

1.08 ± 0.02

1.10 ± 0.06

1.02 ± 0.02

1.24 ± 0.02

 NB-MLV

n.d.

n.d.

n.d.

n.d.

1.24 ± 0.01

 EIAV

0.79 ± 0.02

0.44 ± 0.02

0.27 ± 0.02

0.26 ± 0.03

0.21 ± 0.01

 FFV

n.d.

n.d.

n.d.

n.d.

1.01 ± 0.03

Fv1CAR1

     

 N-MLV

n.d.

n.d.

n.d.

n.d.

1.21 ± 0.07

 B-MLV

n.d.

n.d.

n.d.

n.d.

1.15 ± 0.03

 NB-MLV

n.d.

n.d.

n.d.

n.d.

1.17 ± 0.01

 EIAV

n.d.

n.d.

n.d.

n.d.

1.17 ± 0.09

 FFV

0.95 ± 0.01

0.40 ± 0.04

0.18 ± 0.02

0.18 ± 0.03

0.13 ± 0.01

  1. We arbitrarily divide restriction activity into three levels; Full, shown in bolditalic (restriction ratio ≤0.3), Partial, indicated in italics (0.3 < ratio ≤ 0.7) and None (ratio > 0.7)
  2. Table shows the results from this study using the TYIx-Fv1 inducible vector, with mean and standard deviation values calculated from two independent duplicate experiments. The last column shows results from our previous study using the non-inducible LxIY-Fv1 vector in MDTF cells [8]. n.d., not determined