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Table 2 Restriction activity of various Fv1 mutants against MLVs at different Fv1 expression levels

From: Expression levels of Fv1: effects on retroviral restriction specificities

Vector TGx-Fv1 TGIx-Fv1 TGx-Fv1 TGIx-Fv1 LxIG-Fv1 Activity profilea
Dox (ng/mL) 0 0 1000 1000 0  
Fv1n       
 N-MLV 1.15 ± 0.07 1.22 ± 0.11 1.16 ± 0.04 1.13 ± 0.05 1.07 ± 0.03 D
 B-MLV 1.15 ± 0.09 0.56 ± 0.01 0.24 ± 0.04 0.14 ± 0.02 0.06 ± 0.01 A
 NB-MLV 1.17 ± 0.06 1.21 ± 0.04 1.18 ± 0.04 1.09 ± 0.06 1.08 ± 0.01 D
Fv1b       
 N-MLV 1.02 ± 0.09 0.45 ± 0.09 0.17 ± 0.01 0.13 ± 0.01 0.07 ± 0.02 A
 B-MLV 1.13 ± 0.07 1.12 ± 0.02 1.10 ± 0.06 0.87 ± 0.01 0.63 ± 0.02 C
 NB-MLV 1.21 ± 0.04 1.01 ± 0.07 0.79 ± 0.03 0.35 ± 0.02 0.21 ± 0.03 B
Fv1bbn       
 N-MLV 0.97 ± 0.02 0.38 ± 0.04 0.17 ± 0.06 0.11 ± 0.00 0.06 ± 0.01 A
 B-MLV 1.10 ± 0.02 0.58 ± 0.10 0.29 ± 0.06 0.11 ± 0.02 0.07 ± 0.03 A
 NB-MLV 0.96 ± 0.08 0.33 ± 0.07 0.19 ± 0.04 0.08 ± 0.02 0.08 ± 0.02 A
Fv1nnb       
 N-MLV 1.18 ± 0.06 1.09 ± 0.07 1.07 ± 0.07 0.72 ± 0.10 0.58 ± 0.04 C
 B-MLV 1.13 ± 0.09 0.76 ± 0.03 0.52 ± 0.03 0.14 ± 0.03 0.09 ± 0.02 A
 NB-MLV 1.15 ± 0.10 1.16 ± 0.06 1.07 ± 0.10 1.07 ± 0.13 1.07 ± 0.05 D
Fv1bnn       
 N-MLV 1.14 ± 0.07 0.46 ± 0.01 0.18 ± 0.07 0.12 ± 0.02 0.09 ± 0.06 A
 B-MLV 1.20 ± 0.01 1.04 ± 0.04 0.93 ± 0.05 0.33 ± 0.03 0.12 ± 0.03 B
 NB-MLV 1.21 ± 0.12 1.09 ± 0.10 1.12 ± 0.06 1.02 ± 0.03 0.98 ± 0.05 D
Fv1nbb       
 N-MLV 1.17 ± 0.04 1.17 ± 0.02 1.05 ± 0.07 1.01 ± 0.03 0.93 ± 0.07 D
 B-MLV 1.18 ± 0.04 0.81 ± 0.08 0.66 ± 0.03 0.12 ± 0.05 0.07 ± 0.02 A
 NB-MLV 1.15 ± 0.06 1.09 ± 0.05 1.12 ± 0.11 1.17 ± 0.07 1.10 ± 0.07 D
Fv1nbn       
 N-MLV 1.20 ± 0.05 1.10 ± 0.03 1.12 ± 0.09 1.03 ± 0.07 1.02 ± 0.11 D
 B-MLV 1.12 ± 0.07 0.50 ± 0.04 0.23 ± 0.01 0.09 ± 0.02 0.08 ± 0.03 A
 NB-MLV 1.11 ± 0.02 0.62 ± 0.11 0.33 ± 0.05 0.12 ± 0.03 0.09 ± 0.02 A
Fv1bnb       
 N-MLV 1.05 ± 0.08 0.56 ± 0.06 0.22 ± 0.03 0.10 ± 0.02 0.06 ± 0.01 A
 B-MLV 1.09 ± 0.05 1.13 ± 0.02 1.08 ± 0.08 1.12 ± 0.10 1.11 ± 0.04 D
 NB-MLV 1.13 ± 0.07 1.09 ± 0.09 1.08 ± 0.11 1.09 ± 0.05 1.12 ± 0.08 D
Fv1nn_       
 N-MLV 1.17 ± 0.04 1.15 ± 0.04 1.09 ± 0.07 1.18 ± 0.05 1.10 ± 0.06 D
 B-MLV 1.12 ± 0.11 1.11 ± 0.04 1.07 ± 0.02 0.37 ± 0.05 0.09 ± 0.02 B
 NB-MLV 1.12 ± 0.10 1.11 ± 0.05 1.12 ± 0.13 1.05 ± 0.06 1.11 ± 0.05 D
Fv1bb_       
 N-MLV 1.07 ± 0.17 1.04 ± 0.05 1.07 ± 0.04 0.15 ± 0.04 0.06 ± 0.02 B
 B-MLV 1.14 ± 0.04 1.15 ± 0.05 1.09 ± 0.11 0.77 ± 0.04 0.22 ± 0.01 C
 NB-MLV 1.13 ± 0.03 1.04 ± 0.06 1.07 ± 0.10 0.20 ± 0.01 0.05 ± 0.02 B
  1. We arbitrarily divide restriction activity into three levels; Full, shown in bolditalic (restriction ratio ≤0.3), Partial, indicated in italics (0.3 < ratio ≤ 0.7) and None (ratio > 0.7)
  2. Table shows mean and standard deviation values calculated from three independent restriction experiments carried out in transduced MDTF-R18 cells. Data for Fv1n and Fv1b from Table 1
  3. aClassification into groups of constructs with similar activity profiles. A. Full restriction; B. Full restriction only at elevated concentration; C. Partial restriction at high concentrations; D. No restriction. Full and partial restriction defined as in Table 1