Expression levels of Fv1: effects on retroviral restriction specificities

Li, Wilson; Yap, Melvyn W.; Voss, Vicky; Stoye, Jonathan P.

doi:10.1186/s12977-016-0276-7

Retrovirology

Table 2 Restriction activity of various Fv1 mutants against MLVs at different Fv1 expression levels

From: Expression levels of Fv1: effects on retroviral restriction specificities

Vector	TGx-Fv1	TGIx-Fv1	TGx-Fv1	TGIx-Fv1	LxIG-Fv1	Activity profile^a
Dox (ng/mL)	0	0	1000	1000	0
Fv1ⁿ
N-MLV	1.15 ± 0.07	1.22 ± 0.11	1.16 ± 0.04	1.13 ± 0.05	1.07 ± 0.03	D
B-MLV	1.15 ± 0.09	0.56 ± 0.01	*0.24* ± *0.04*	*0.14* ± *0.02*	*0.06* ± *0.01*	A
NB-MLV	1.17 ± 0.06	1.21 ± 0.04	1.18 ± 0.04	1.09 ± 0.06	1.08 ± 0.01	D
Fv1^b
N-MLV	1.02 ± 0.09	0.45 ± 0.09	*0.17* ± *0.01*	*0.13* ± *0.01*	*0.07* ± *0.02*	A
B-MLV	1.13 ± 0.07	1.12 ± 0.02	1.10 ± 0.06	0.87 ± 0.01	0.63 ± 0.02	C
NB-MLV	1.21 ± 0.04	1.01 ± 0.07	0.79 ± 0.03	0.35 ± 0.02	*0.21* ± *0.03*	B
Fv1bbn
N-MLV	0.97 ± 0.02	0.38 ± 0.04	*0.17* ± *0.06*	*0.11* ± *0.00*	*0.06* ± *0.01*	A
B-MLV	1.10 ± 0.02	0.58 ± 0.10	*0.29* ± *0.06*	*0.11* ± *0.02*	*0.07* ± *0.03*	A
NB-MLV	0.96 ± 0.08	0.33 ± 0.07	*0.19* ± *0.04*	*0.08* ± *0.02*	*0.08* ± *0.02*	A
Fv1nnb
N-MLV	1.18 ± 0.06	1.09 ± 0.07	1.07 ± 0.07	0.72 ± 0.10	0.58 ± 0.04	C
B-MLV	1.13 ± 0.09	0.76 ± 0.03	0.52 ± 0.03	*0.14* ± *0.03*	*0.09* ± *0.02*	A
NB-MLV	1.15 ± 0.10	1.16 ± 0.06	1.07 ± 0.10	1.07 ± 0.13	1.07 ± 0.05	D
Fv1bnn
N-MLV	1.14 ± 0.07	0.46 ± 0.01	*0.18* ± *0.07*	*0.12* ± *0.02*	*0.09* ± *0.06*	A
B-MLV	1.20 ± 0.01	1.04 ± 0.04	0.93 ± 0.05	0.33 ± 0.03	*0.12* ± *0.03*	B
NB-MLV	1.21 ± 0.12	1.09 ± 0.10	1.12 ± 0.06	1.02 ± 0.03	0.98 ± 0.05	D
Fv1nbb
N-MLV	1.17 ± 0.04	1.17 ± 0.02	1.05 ± 0.07	1.01 ± 0.03	0.93 ± 0.07	D
B-MLV	1.18 ± 0.04	0.81 ± 0.08	0.66 ± 0.03	*0.12* ± *0.05*	*0.07* ± *0.02*	A
NB-MLV	1.15 ± 0.06	1.09 ± 0.05	1.12 ± 0.11	1.17 ± 0.07	1.10 ± 0.07	D
Fv1nbn
N-MLV	1.20 ± 0.05	1.10 ± 0.03	1.12 ± 0.09	1.03 ± 0.07	1.02 ± 0.11	D
B-MLV	1.12 ± 0.07	0.50 ± 0.04	*0.23* ± *0.01*	*0.09* ± *0.02*	*0.08* ± *0.03*	A
NB-MLV	1.11 ± 0.02	0.62 ± 0.11	0.33 ± 0.05	*0.12* ± *0.03*	*0.09* ± *0.02*	A
Fv1bnb
N-MLV	1.05 ± 0.08	0.56 ± 0.06	*0.22* ± *0.03*	*0.10* ± *0.02*	*0.06* ± *0.01*	A
B-MLV	1.09 ± 0.05	1.13 ± 0.02	1.08 ± 0.08	1.12 ± 0.10	1.11 ± 0.04	D
NB-MLV	1.13 ± 0.07	1.09 ± 0.09	1.08 ± 0.11	1.09 ± 0.05	1.12 ± 0.08	D
Fv1nn_
N-MLV	1.17 ± 0.04	1.15 ± 0.04	1.09 ± 0.07	1.18 ± 0.05	1.10 ± 0.06	D
B-MLV	1.12 ± 0.11	1.11 ± 0.04	1.07 ± 0.02	0.37 ± 0.05	*0.09* ± *0.02*	B
NB-MLV	1.12 ± 0.10	1.11 ± 0.05	1.12 ± 0.13	1.05 ± 0.06	1.11 ± 0.05	D
Fv1bb_
N-MLV	1.07 ± 0.17	1.04 ± 0.05	1.07 ± 0.04	*0.15* ± *0.04*	*0.06* ± *0.02*	B
B-MLV	1.14 ± 0.04	1.15 ± 0.05	1.09 ± 0.11	0.77 ± 0.04	*0.22* ± *0.01*	C
NB-MLV	1.13 ± 0.03	1.04 ± 0.06	1.07 ± 0.10	*0.20* ± *0.01*	*0.05* ± *0.02*	B

We arbitrarily divide restriction activity into three levels; Full, shown in bolditalic (restriction ratio ≤0.3), Partial, indicated in italics (0.3 < ratio ≤ 0.7) and None (ratio > 0.7)
Table shows mean and standard deviation values calculated from three independent restriction experiments carried out in transduced MDTF-R18 cells. Data for Fv1ⁿ and Fv1^b from Table 1
^aClassification into groups of constructs with similar activity profiles. A. Full restriction; B. Full restriction only at elevated concentration; C. Partial restriction at high concentrations; D. No restriction. Full and partial restriction defined as in Table 1

Back to article page

ISSN: 1742-4690

Contact us

Submission enquiries: journalsubmissions@springernature.com