Expression levels of Fv1: effects on retroviral restriction specificities

Retrovirology

Table 1 Restriction activity of Fv1ⁿ and Fv1^b against MLVs at different expression levels

Vector	TGx-Fv1		TGIx-Fv1		LxIG-Fv1
Dox (ng/mL)	0	1000	0	1000	0
Fv1ⁿ
N-MLV	1.15 ± 0.07	1.16 ± 0.04	1.22 ± 0.11	1.13 ± 0.05	1.07 ± 0.03
B-MLV	1.15 ± 0.09	*0.24* ± *0.04*	0.56 ± 0.01	*0.14* ± *0.02*	*0.06* ± *0.01*
NB-MLV	1.17 ± 0.06	1.18 ± 0.04	1.21 ± 0.04	1.09 ± 0.06	1.08 ± 0.01
Fv1^b
N-MLV	1.02 ± 0.09	*0.17* ± *0.01*	0.45 ± 0.09	*0.13* ± *0.01*	*0.07* ± *0.02*
B-MLV	1.13 ± 0.07	1.10 ± 0.06	1.12 ± 0.02	0.87 ± 0.01	0.63 ± 0.02
NB-MLV	1.21 ± 0.04	0.79 ± 0.03	1.01 ± 0.07	0.35 ± 0.02	*0.21* ± 0.03

Table shows mean and standard deviation values calculated from three independent restriction experiments carried out in transduced MDTF-R18 cells. A lower restriction ratio indicates stronger inhibition by Fv1. We arbitrarily divide restriction activity into three levels; Full, shown in bolditalic (restriction ratio ≤0.3), Partial, indicated in italics (0.3 < ratio ≤ 0.7) and None (ratio > 0.7)

ISSN: 1742-4690