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Fig. 4 | Retrovirology

Fig. 4

From: Expression levels of Fv1: effects on retroviral restriction specificities

Fig. 4

Comparison of endogenous and overexpression levels of Fv1n and Fv1b. a Quantitative western blot analysis of Fv1 in N-3T3, B-3T3 and MDTF transduced with LxIG-Fv1 deliver vectors containing wild type or mutant Fv1 ORF. Fv1 was detected using an anti-Fv1 antibody and the LI-COR Odyssey infrared fluorescent western blot system. Quantities of Fv1 in lysate samples were interpolated from a standard curve generated using fluorescent signals from known quantities of purified Fv1NTD protein loaded on the same gel. Numbers under the blot indicate the quantities of Fv1 in each sample relative to that in the N-3T3 sample. b A bar chart showing the mean and mean deviation values from two independent quantitation experiments

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