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Fig. 2 | Retrovirology

Fig. 2

From: Expression levels of Fv1: effects on retroviral restriction specificities

Fig. 2

Expression levels of Fv1n and Fv1b from inducible and non-inducible retroviral vectors. a Quantitative western blot analysis of Fv1 in N-3T3 cells, B-3T3 cells, and in MDTF-R18 cells transduced with either non-inducible or inducible vectors expressing Fv1 (MOI 0.7). Fv1 was detected using an anti-Fv1 antibody and the LI-COR Odyssey infrared fluorescent western blot system. 1000 ng/mL doxycycline was added to culture media for induced samples. Quantities of Fv1 in lysate samples were interpolated from a standard curve generated using fluorescent signals from known quantities of purified Fv1NTD protein loaded on the same gel. Numbers under the blot indicate the quantities of Fv1 in each sample relative to that in the N-3T3 sample. b Quantities of Fv1 in transduced MDTF-R18 and B-3T3, relative to that of N-3T3. Data from Additional file 1 are shown as a grouped scatter plot. Mean values are represented by horizontal lines, while individual results are plotted as symbols: filled circle, endogenous Fv1 from N-3T3 or B-3T3 cells; open square, TGx-Fv1 without dox; filled square, TGx-Fv1 with 1000 ng/mL dox; open triangle, TGIx-Fv1 without dox; filled triangle, TGIx-Fv1 with 1000 ng/mL dox; filled diamond, LxIG-Fv1 without dox

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