Fig. 9

SIVmac and HIV-2 escape inhibition by FcaA3Z2Z3. a, b 293T cells were transfected with expression plasmids for a SIVmacΔvif-Luc (SIVmacΔvif) or SIVmac-Luc (SIVmac WT) or b HIV-2Δvif-Luc (HIV-2Δvif) or HIV-2Δvif-Luc + HIV-2 Vif (HIV-2 WT), together with expression plasmids for HsaA3G or FcaA3s, pcDNA3.1 (+) was used as a control (vector). Reporter virus infectivity was determined by quantification of luciferase activity in 293T cells transduced with vector particles after normalizing for reverse transcriptase activity. Luc luciferase. c Lysates of SIVmac producer cells were used to detect the expression of FcaA3s and SIVmac capsid by anti-HA and anti-p27 antibodies, respectively. SIVmac Vif cannot be detected because of the unavailability of a suitable antibody. d Lysates of HIV-2 producer cells were used to detect the expression of FcaA3s and HIV-2 Vif by anti-HA and anti-V5 antibody, respectively. BIR, BOB, SHO and VAN represent FcaA3Z2Z3s including polymorphic sequences of exon 4 of four different Felis catus breeding lines: BIR Birman, BOB Japanese Bobtail, SHO British Shorthair, VAN Turkish Van. Asterisks represent statistically significant differences: ***p < 0.001; **0.001 < p < 0.01; *0.01 < p < 0.05; ^ns p > 0.05 (Dunnett t test)