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Fig. 6 | Retrovirology

Fig. 6

From: Determinants of FIV and HIV Vif sensitivity of feline APOBEC3 restriction factors

Fig. 6

Differential binding of FIV Vif to wild-type and mutant feline A3s. a Expression plasmids for FcaA3Z3s wild-type and mutants (all with HA-tag) and FIV Vif-TLQAAA (V5 tag) were co-transfected into 293T cells. The proteins were immunoprecipitated by α-HA beads and analyzed by immunoblots using anti-HA and anti-V5 antibodies. b Expression plasmids for FcaA3s (FcaA3Z2, FcaA3Z2.DH-YN, FcaA3Z2Z3 and FcaA3Z2Z3-M, all with HA-tag) and FIV Vif-TLQAAA (V5-tag) and were co-transfected into 293T cells, pcDNA3.1 (+) served as an A3-free control. 48 h later, cells were harvested, proteins were immunoprecipitated by α-HA beads. The FcaA3s and FIV Vif proteins were detected by anti-HA and anti-V5 antibodies, respectively

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