Fig. 1
From: Determinants of FIV and HIV Vif sensitivity of feline APOBEC3 restriction factors
The interaction of feline APOBEC3s with FIV Vif. a Representation of APOBEC3 (A3) genes in the genome of Felis catus, chromosome (chr.) B4. Coding regions of the A3 genes (A3Z2a, A3Z2b, A3Z2c, A3Z3) shown as arrows. Red rectangle exon 2 of A3Z3 that is untranslated in the A3Z3 mRNA, however translated (“linker domain”) in readthrough transcripts A3Z2bZ3 and A3Z2cZ3. Spliced-out introns (dashed lines) are indicated, mRNAs for A3Z2s and A3Z3 not shown. For details, see references [4, 36]. b 293T cells were transfected with expression plasmids for FcaA3Z2a, FcaA3Z2b, FcaA3Z2c, FcaA3Z3, FcaA3Z2bZ3 and FcaA3Z2cZ3 together with HIV-1, SIVmac, HIV-2 and SIVsmm or FIV Vif or no Vif (replaced by pcDNA3.1). FcaA3s, Vifs and tubulin were visualized by immunoblot using anti-HA, anti-V5 and anti-tubulin antibodies. c Immunoprecipitation of E. coli expressed FcaA3Z2 and -Z3 (GST fusion proteins) after mixing with 293T-derived FIV Vif (Vif-V5). Asterisk indicates the impression of GST blob on the blot and Dagger denotes for a possible degradation product of FcaA3Z3