Association of mutations in V3/C3 domain with enhanced sensitivity of HIV-1 clade C primary envelopes to autologous broadly neutralizing plasma antibodies

Table 3 Effect of depletion of G37080 BCN plasma antibodies on autologous virus neutralization

G37080 depleted plasma (fold reduction in ID₅₀)
Env-pseudotyped viruses	gp140 Trimer (BG505-SOSIP.664)	gp120 Monomer (4-2.J41)	MPER (C1C peptide)	References
HVTR-PG80v2.eJ7	>150.0	80.0	1.00	This study
HVTR-PG80v2.eJ38 (v2.eJ7V3/C3)	>70.0	>10.0	1.10	This study
HVTR-PG80v1.eJ7	>10.03	0.9	1.12	[13]
HVTR-PG80v1.eJ19	>15.60	0.5	1.18	[13]
92BR020	>35.08	1.1	1.34	[13]

Fold reduction in virus neutralization was obtained by comparing the neutralization titer (ID₅₀ values) of Env-pseudotyped viruses against undepleted and depleted G37080 plasma. ID₅₀ values are reciprocal dilutions at which the undepleted and depleted plasma conferred 50 % neutralization of the Env-pseudotyped viruses in TZM-bl cells. ‘v1’ and ‘v2’ refers to Envs obtained from visit 1 and visit 2 plasma samples

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