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Fig. 3 | Retrovirology

Fig. 3

From: In vivo analysis of the effect of panobinostat on cell-associated HIV RNA and DNA levels and latent HIV infection

Fig. 3

Outline of panobinostat treatment of HIV-infected, ART-suppressed BLT mice. a Experimental outline. BLT mice were infected intravenously with HIV-1JR-CSF. Starting at 2 weeks post exposure, mice were bled weekly for plasma viral load and flow cytometry analysis. Starting at 3 weeks post exposure, mice were administered antiretroviral therapy (ART) consisting of tenofovir disoproxil fumarate, emtricitabine, and raltegravir (light blue shading). After 6 weeks of ART, one group of mice was administered panobinostat at a dose of 2 mg/kg intraperitoneally (red circles, n = 9) or vehicle (blue squares, n = 4), twice a week for 2 weeks, in addition to ART (light red shading). At the end of the experiment, mice were harvested, and cells were isolated for real-time PCR analysis and flow cytometry analysis. b Plasma viral load was measured by quantitative real-time PCR (limit of detection = 750 copies/ml, dashed line), and c  %CD4+ T cells was measured by flow cytometry. Mean values and SEM plotted

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