Fig. 1
Effects of R263K and H51Y/R263K in combination with different RTI substitutions on viral infectivity in TZM-bl reporter cells. Expressed luciferase levels were measured at 48 h.p.i. and normalized to relative p24 levels. Best-fit curves and data for EC50 values, 95 % confidence intervals and fold changes (FC) relative to single RT mutations alone are showed in the insets: a K65R series including NL4.3K65R, NL4.3R263K-K65R, and NL4.3H51Y/R263K-K65R. b L74V series including NL4.3L74V, NL4.3R263K-L74V, and NL4.3H51Y/R263K-L74V. c K103N series including NL4.3K103N, NL4.3R263K-K103N, and NL4.3H51Y/R263K-K103N. d E138K series including NL4.3E138K, NL4.3R263K-E138K, and NL4.3H51Y/R263K-E138K. e M184I series including NL4.3M184I, NL4.3R263K-M184I, and NL4.3H51Y/R263K-M184I. f M184V series including NL4.3M184V, NL4.3R263K-M184V, and NL4.3H51Y/R263K-M184V. The FC value of each singly mutated RT virus was arbitrarily set at 1. Error bars indicate mean ± standard deviation (SD). Asterisk and dollar sign indicate statistically significant difference from single RT mutants alone and from the R263K-containing viruses, respectively (Student’s t test, P < 0.05)