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Fig. 5 | Retrovirology

Fig. 5

From: Absolute quantification of HTLV-1 basic leucine zipper factor (HBZ) protein and its plasma antibody in HTLV-1 infected individuals with different clinical status

Fig. 5

Quantification of HBZ protein expression levels in HTLV-1-infected T-cell lines. The absolute intracellular concentration of an endogenous HBZ protein in HTLV-1-infected T-cell lines was analyzed by a novel HBZ sandwich ELISA. a Western blot image of twofold serially diluted recombinant HBZ protein probed with anti-HBZ mAb (clone #91-1). b A representative standard curve for the ELISA comprised of twofold serial dilutions of recombinant HBZ protein (from 4000 to 3.91 ng/mL). c Absolute quantification of HBZ protein in HTLV-1-infected T-cell lines. Results are presented as mean ± SD of three independent experiments with duplicate wells

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